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m is one/400 of that measured for the wild style enzyme. These results ensure the significance with the job of H251 from the binding of substrate in the active website of NagA. The homologous residue was identified to connect with d-glucosamine-six-phosphate while in the structure of NagA from B. subtilis
a may be the acid dissociation constant. pH-level profiles that confirmed the lack of catalytic action at high and small values of pH had been suit to equation four, the place K
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-acetyl-l-aspartate is a very good substrate for aspartoacylase from human Mind (31). The kinetic parameters with the hydrolysis of six with NagA are mentioned in Table 2 This substrate is hydrolyzed in a substantially a lot quicker fee than may be the corresponding N
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